Explore how PCR technology revolutionized laboratory testing and why it remains the most accurate diagnostic method.
What is PCR Testing?
Polymerase Chain Reaction (PCR) is a revolutionary technique that allows scientists to amplify small segments of DNA, making it possible to detect even tiny amounts of genetic material.
How PCR Works
The PCR process involves three main steps that are repeated multiple times:
- Denaturation: The DNA sample is heated to separate the double strand
- Annealing: Primers attach to specific target sequences
- Extension: DNA polymerase creates new copies of the target DNA
After 30-40 cycles, millions of copies of the target DNA are created, making detection possible.
Applications in Healthcare
PCR testing is used for:
- Infectious disease detection
- Genetic disorder screening
- Cancer marker identification
- Pathogen identification
Why PCR is the Gold Standard
PCR offers unmatched sensitivity and specificity, capable of detecting a single copy of DNA in a sample. This makes it invaluable for early disease detection and accurate diagnosis.
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Written by
Lab Director Team